Water with Heat
Room Temperature
Directions: Dissolve 24.2 g in 1L distilled water until evenly dispersed. Heat and stir until completely dissolved. Sterilize by autoclaving for 15 minutes. After autoclaving, place medium on a slant and allow to solidify.
Formulation per Liter:
Agar 15.0g
Ammonium Dihydrogen Phosphate 1.0g
Bromothymol Blue 0.08g
Magnesium Sulfate 0.2g
Potassium Phosphate Dibasic 1.0g
Sodium Chloride 5.0g
Sodium Citrate 2.0g
Final pH (25°C) 6.9±0.2
4.4 lbs
4.88 x 10.25 x 4.88
Research or further manufacturing use only, not for food or drug use.
